Preface |
|
ix | |
Foreword to Current Protocols in Food Analytical Chemistry |
|
xi | |
Contributors |
|
xii | |
|
|
1 | (70) |
|
Gravimetric Measurements of Water |
|
|
5 | (30) |
|
Gravimetric Determination of Water by Drying and Weighing |
|
|
7 | (6) |
|
|
13 | (4) |
|
Application of Low-Resolution NMR for Simultaneous Moisture and Oil Determination in Food (Oilseeds) |
|
|
17 | (12) |
|
Traditional Indirect Methods for Estimation of Water Content: Measurement of Brix |
|
|
29 | (6) |
|
Vapor Pressure Measurements of Water |
|
|
35 | (36) |
|
Factors to Consider When Estimating Water Vapor Pressure |
|
|
37 | (4) |
|
Dew-Point Method for the Determination of Water Activity |
|
|
41 | (10) |
|
Measurement of Water Activity Using Isopiestic Method |
|
|
51 | (10) |
|
Direct Manometric Determination of Vapor Pressure |
|
|
61 | (6) |
|
Measurement of Water Activity by Electronic Sensors |
|
|
67 | (4) |
|
|
71 | (254) |
|
Measurement of Protein Content |
|
|
73 | (50) |
|
The Colorimetric Detection and Quantitation of Total Protein |
|
|
77 | (28) |
|
Determination of Total Nitrogen |
|
|
105 | (10) |
|
Spectrophotometric Determination of Protein Concentration |
|
|
115 | (8) |
|
Biochemical Compositional Analyses of Proteins |
|
|
123 | (32) |
|
Analyses of Protein Quality |
|
|
125 | (16) |
|
Evaluation of the Progress of Protein Hydrolysis |
|
|
141 | (14) |
|
Characterization of Proteins |
|
|
155 | (112) |
|
|
157 | (28) |
|
Electroblotting from Polyacrylamide Gels |
|
|
185 | (14) |
|
Detection of Proteins on Blot Membranes |
|
|
199 | (8) |
|
|
207 | (12) |
|
Determining the CD Spectrum of a Protein |
|
|
219 | (26) |
|
Determining the Fluorescence Spectrum of a Protein |
|
|
245 | (22) |
|
|
267 | (22) |
|
Overview of Protein Purification and Characterization |
|
|
269 | (10) |
|
Overview of Conventional Chromatography |
|
|
279 | (10) |
|
Functionality of Proteins |
|
|
289 | (36) |
|
Measurement of Functional Properties: Overview of Protein Functionality Testing |
|
|
291 | (10) |
|
Measurement of Protein Hydrophobicity |
|
|
301 | (14) |
|
Water Retention Properties of Solid Foods |
|
|
315 | (10) |
|
|
325 | (94) |
|
Strategies for Enzyme Activity Measurements |
|
|
329 | (20) |
|
Expression and Measurement of Enzyme Activity |
|
|
331 | (4) |
|
Detecting Enzyme Activity: A Case Study of Polygalacturonase |
|
|
335 | (14) |
|
|
349 | (20) |
|
Activity Measurements of Proteinases Using Synthetic Substrates |
|
|
351 | (8) |
|
Peptidase Activity Assays Using Protein Substrates |
|
|
359 | (10) |
|
|
369 | (16) |
|
|
371 | (14) |
|
|
385 | (34) |
|
Polarographic and Spectrophotometric Assay of Diphenol Oxidases (Polyphenol Oxidase) |
|
|
387 | (16) |
|
Analysis of Lipoxygenase Activity and Products |
|
|
403 | (16) |
|
|
419 | (228) |
|
|
423 | (90) |
|
Extraction and Measurement of Total Lipids |
|
|
425 | (12) |
|
Analysis of Fatty Acids in Food Lipids |
|
|
437 | (16) |
|
|
453 | (14) |
|
|
467 | (12) |
|
Analysis of Tocopherols and Tocotrienols |
|
|
479 | (12) |
|
Quantitation of Lipid Classes by Thin-Layer Chromatography with Flame lonization Detection |
|
|
491 | (14) |
|
Infrared Spectroscopic Determination of Total Trans Fatty Acids |
|
|
505 | (8) |
|
Lipid Oxidation/Stability |
|
|
513 | (52) |
|
Measurement of Primary Lipid Oxidation Products |
|
|
515 | (16) |
|
Chromatographic Analysis of Secondary Lipid Oxidation Products |
|
|
531 | (10) |
|
Assessment of Oxidative Stability for Lipids |
|
|
541 | (6) |
|
Spectrophotometric Measurement of Secondary Lipid Oxidation Products |
|
|
547 | (18) |
|
Physical Properties of Lipids |
|
|
565 | (82) |
|
Determination of Solid Fat Content by Nuclear Magnetic Resonance |
|
|
567 | (8) |
|
Lipid Crystal Characterization |
|
|
575 | (6) |
|
Emulsion Droplet Size Determination |
|
|
581 | (10) |
|
Emulsion Stability Determination |
|
|
591 | (18) |
|
Key Concepts of Interfacial Properties in Food Chemistry |
|
|
609 | (22) |
|
Static and Dynamic Interfacial Tension Analysis |
|
|
631 | (16) |
|
|
647 | (110) |
|
Mono-and Oligosaccharides |
|
|
651 | (20) |
|
Colorimetric Quantification of Carbohydrates |
|
|
653 | (8) |
|
HPLC of Mono-and Disaccharids Using Refractive Index Detection |
|
|
661 | (10) |
|
Starch and Starch Derivatives |
|
|
671 | (24) |
|
Overview of Laboratory Isolation of Starch from Plant Materials |
|
|
673 | (6) |
|
Enzymatic Quantitation of Total Starch in Plant Products |
|
|
679 | (10) |
|
Determination of Total Amylose Content of Starch |
|
|
689 | (6) |
|
Cell Wall Polysaccharides |
|
|
695 | (62) |
|
Isolation of Plant Cell Walls and Fractionation of Cell Wall Polysaccharides |
|
|
697 | (24) |
|
Determination of Neutural Sugars by Gas Chromatography of Their Alditol Acetates |
|
|
721 | (14) |
|
Determination of the Uronic Acid Content of Plant Cell Walls Using a Colorimetric Assay |
|
|
735 | (4) |
|
Determining the Degree of Methylation and Acetylation of Pectin |
|
|
739 | (6) |
|
Quantitative Determination of β-Glucan Content |
|
|
745 | (12) |
Index |
|
757 | |