Handbook of Food Analytical Chemistry, Volumes 1 and 2

by ; ; ; ; ; ; ; ;
Edition: 1st
Format: Hardcover
Pub. Date: 2004-12-27
Publisher(s): Wiley-Interscience
List Price: $538.60

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Summary

Emphasizing effective, state-of-the-art methodology, the Handbook of Food Analytical Chemistry represents the most comprehensive resource of its kind. Each section includes detailed instructions with annotated advisory comments, critical and troubleshooting notes, key references with annotations, time considerations, and anticipated results. In addition, useful appendices feature common abbreviations; laboratory stock solutions, equipment, and guidelines; and commonly used techniques, including relevant notes on mass spectrometry. It is an indispensable reference for all scientists, technicians, and students in food science.

Author Biography

Ronald E. Wrolstad, PhD,?is a Distinguised Professor in the Department of Food Science & Technology at Oregon State University.

Eric A. Decker, PbD,?is a Professor in the Department of Food Science at the University of Massachusetts, Amherst. He is an author, with Cameron Faustman and Clemente J. Lopez-Bote of Antioxidants in Muscle Foods: Nutritional Strategies to Improve Quality, also published by Wiley.

Steven J. Schwartz, PhD,?is a Professor in the Department of Food Science & Technology at The Ohio State University. Peter Sporns, PhD,?is a Professor in the Department of Agricultural, Food, and Nutritional Science and is the Associate Chair of the graduate program in that department at the University of Alberta, Canada.

Table of Contents

Preface ix
Foreword to Current Protocols in Food Analytical Chemistry xi
Contributors xii
Water
1(70)
Gravimetric Measurements of Water
5(30)
Gravimetric Determination of Water by Drying and Weighing
7(6)
Karl Fischer Titration
13(4)
Application of Low-Resolution NMR for Simultaneous Moisture and Oil Determination in Food (Oilseeds)
17(12)
Traditional Indirect Methods for Estimation of Water Content: Measurement of Brix
29(6)
Vapor Pressure Measurements of Water
35(36)
Factors to Consider When Estimating Water Vapor Pressure
37(4)
Dew-Point Method for the Determination of Water Activity
41(10)
Measurement of Water Activity Using Isopiestic Method
51(10)
Direct Manometric Determination of Vapor Pressure
61(6)
Measurement of Water Activity by Electronic Sensors
67(4)
Proteins
71(254)
Measurement of Protein Content
73(50)
The Colorimetric Detection and Quantitation of Total Protein
77(28)
Determination of Total Nitrogen
105(10)
Spectrophotometric Determination of Protein Concentration
115(8)
Biochemical Compositional Analyses of Proteins
123(32)
Analyses of Protein Quality
125(16)
Evaluation of the Progress of Protein Hydrolysis
141(14)
Characterization of Proteins
155(112)
Electrophoresis Analysis
157(28)
Electroblotting from Polyacrylamide Gels
185(14)
Detection of Proteins on Blot Membranes
199(8)
Immunoblot Detection
207(12)
Determining the CD Spectrum of a Protein
219(26)
Determining the Fluorescence Spectrum of a Protein
245(22)
Purification of Proteins
267(22)
Overview of Protein Purification and Characterization
269(10)
Overview of Conventional Chromatography
279(10)
Functionality of Proteins
289(36)
Measurement of Functional Properties: Overview of Protein Functionality Testing
291(10)
Measurement of Protein Hydrophobicity
301(14)
Water Retention Properties of Solid Foods
315(10)
Enzymes
325(94)
Strategies for Enzyme Activity Measurements
329(20)
Expression and Measurement of Enzyme Activity
331(4)
Detecting Enzyme Activity: A Case Study of Polygalacturonase
335(14)
Proteolytic Enzymes
349(20)
Activity Measurements of Proteinases Using Synthetic Substrates
351(8)
Peptidase Activity Assays Using Protein Substrates
359(10)
Lipolytic Enzymes
369(16)
Lipase Assays
371(14)
Oxidoreductases
385(34)
Polarographic and Spectrophotometric Assay of Diphenol Oxidases (Polyphenol Oxidase)
387(16)
Analysis of Lipoxygenase Activity and Products
403(16)
Lipids
419(228)
Lipid Composition
423(90)
Extraction and Measurement of Total Lipids
425(12)
Analysis of Fatty Acids in Food Lipids
437(16)
Cholesterol
453(14)
Oil Quality Indices
467(12)
Analysis of Tocopherols and Tocotrienols
479(12)
Quantitation of Lipid Classes by Thin-Layer Chromatography with Flame Ionization Detection
491(14)
Infrared Spectroscopic Determination of Total Trans Fatty Acids
505(8)
Lipid Oxidation/Stability
513(52)
Measurement of Primary Lipid Oxidation Products
515(16)
Chromatographic Analysis of Secondary Lipid Oxidation Products
531(10)
Assessment of Oxidative Stability for Lipids
541(6)
Spectrophotometric Measurement of Secondary Lipid Oxidation Products
547(18)
Physical Properties of Lipids
565(82)
Determination of Solid Fat Content by Nuclear Magnetic Resonance
567(8)
Lipid Crystal Characterization
575(6)
Emulsion Droplet Size Determination
581(10)
Emulsion Stability Determination
591(18)
Key Concepts of Interfacial Properties in Food Chemistry
609(22)
Static and Dynamic Interfacial Tension Analysis
631(16)
Carbohydrates
647(110)
Mono- and Oligosaccharides
651(20)
Colorimetric Quantification of Carbohydrates
653(8)
HPLC of Mono- and Disaccharides Using Refractive Index Detection
661(10)
Starch and Starch Derivatives
671(24)
Overview of Laboratory Isolation of Starch from Plant Materials
673(6)
Enzymatic Quantitation of Total Starch in Plant Products
679(10)
Determination of Total Amylose Content of Starch
689(6)
Cell Wall Polysaccharides
695(62)
Isolation of Plant Cell Walls and Fractionation of Cell Wall Polysaccharides
697(24)
Determination of Neutral Sugars by Gas Chromatography of Their Alditol Acetates
721(14)
Determination of the Uronic Acid Content of Plant Cell Walls Using a Colorimetric Assay
735(4)
Determining the Degree of Methylation and Acetylation of Pectin
739(6)
Quantitative Determination of β-Glucan Content
745(12)
Index 757
Preface ix
Foreword to Current Protocols in Food Analytical Chemistry xi
Contributors xii
Pigments and Colorants
1(216)
Anthocyanins
5(66)
Extraction, Isolation, and Purification of Anthocyanins
7(12)
Characterization and Measurement of Anthocyanins by UV-Visible Spectroscopy
19(14)
Separation and Characterization of Anthocyanins by HPLC
33(14)
Characterization of Anthocyanins by NMR
47(24)
Carotenoids
71(50)
Extraction, Isolation, and Purification of Carotenoids
73(8)
Detection and Measurement of Carotenoids by UV/VIS Spectrophotometry
81(10)
Chromatographic Techniques for Carotenoid Separation
91(16)
Mass Spectrometry of Carotenoids
107(14)
Miscellaneous Colorants
121(32)
Betalains
123(8)
Spectrophotometric and Reflectance Measurements of Pigments of Cooked and Cured Meats
131(8)
Measurement of Discoloration in Fresh Meat
139(14)
Chlorophylls
153(48)
Overview of Chlorophylls in Foods
155(10)
Extraction of Photosynthetic Tissues: Chlorophylls and Carotenoids
165(6)
Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy
171(8)
Chromatographic Separation of Chlorophylls
179(12)
Mass Spectrometry of Chlorophylls
191(10)
Strategies for Measurement of Colors and Pigments
201(16)
Overview of Color Analysis
203(14)
Flavors
217(146)
Smell Chemicals
223(118)
Direct Sampling
225(10)
Isolation and Concentration of Aroma Compounds
235(10)
Identification and Quantitation of Aroma Compounds
245(12)
Stereodifferentiation of Chiral Odorants Using High-Resolution Gas Chromatography
257(20)
Analysis of Citrus Oils
277(24)
Solid-Phase Microextraction for Flavor Analysis
301(12)
Simulation of Mouth Conditions for Flavor Analysis
313(16)
Gas Chromatography/Olfactometry
329(12)
Acid Tastants
341(22)
Titratable Activity of Acid Tastants
343(8)
Liquid Chromatography of Nonvolatile Acids
351(12)
Texture/Rheology
363(94)
Viscosity of Liquids, Solutions, and Fine Suspensions
367(28)
Overview of Viscosity and Its Characterization
369(6)
Measuring the Viscosity of Non-Newtonian Fluids
375(10)
Viscosity Determination of Pure Liquids, Solutions, and Serums Using Capillary Viscometry
385(6)
Measuring Consistency of Juices and Pastes
391(4)
Compressive Measurements of Solids and Semi-Solids
395(30)
General Compressive Measurements
397(8)
Textural Measurements with Special Fixtures
405(12)
Texture Profile Analysis
417(8)
Viscoelasticity of Suspensions and Gels
425(32)
Dynamic or Oscillatory Testing of Complex Fluids
427(12)
Measurement of Gel Rheology: Dynamic Tests
439(10)
Creep and Stress Relaxation: Step-Change Experiments
449(8)
Bioactive Food Components
457(106)
Polyphenolics
461(2)
Determination of Total Phenolics
463(8)
Extraction and Isolation of Polyphenolics
471(12)
HPLC Separation of Polyphenolics
483(16)
Proanthocyanidins: Extraction, Purification, and Determination of Subunit Composition by HPLC
499(12)
Identification of Flavonol Glycosides Using MALDI-MS
511(8)
Analysis of Isoflavones in Soy Foods
519(18)
APPENDICES AND INDEXES
Appendices
537(26)
A.1 Abbreviations and Useful Data
539(1)
A Abbreviations Used in This Manual
539(4)
A.2 Laboratory Stock Solutions, Equipment, and Guidelines
543(1)
A Common Buffers and Stock Solutions
543(8)
B Laboratory Safety
551(2)
C Standard Laboratory Equipment
553(2)
A.3 Commonly Used Techniques
555(1)
A Introduction to Mass Spectrometry for Food Chemistry
555(8)
Suppliers Appendix 563(34)
Index 597

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